Histopathology User Manual Page 8 of 12 SOR 007 Version 1.10 This copy is uncontrolled unless printed on “controlled” Paper. 3. Histology Procedure Fixation 5. PROCEDURE: 1. 2. This textbook deals with basic histopathology techniques: Introduction and examination of tissue, fixation and fixatives, tissue processing and … Choose appropriate inpatient surgical request card or outpatient histopathology specimen requisition and fill in completely (if applicable). Deparaffinize and hydrate to distilled water. *2% Chromic acid, microwave Hi power, 45 seconds, allow to stand for 5 minutes. 9 HISTOPATHOLOGY LABORATORY Histopathology laboratory is a unit performing clinical activity associated with diagnosis during and post-surgery. Hence, the book is titled as Histopathology Techniques and its Management. 1% Sodium metabisulfite, 1 minute, room temperature. I. reagents used within the histology laboratory are available both within the laboratory and in the preparation room (C222). HISTOLOGY AND CYTOLOGY 51 MODULE Microtome Histology and Cytology Notes Fig. 9.1: Rotary Microtome Fig. histopathology is the only way to diagnose infections caused by L. loboi or Rhinosporidium seeberi [Figure 2] as cultivation techniques have to date been unsuccessful. Open biopsies are sent fresh on saline dampened gauze. 4. The lab examines tissue materials, based on which an emergency diagnosis is needed during surgery (i.e. 1. Histology Procedure Fixation Tissue Processing a. Dehydration b. a. II. Clearing c. Infilteration 7. Tissue Fixation Tissues are fixed: •To preserve cells and tissues constituents •To prevent their degradation Bouin’s fixative/formalin for 24 hrs (with fixative’s volume 20 times greater) 6. Wash in tap water, rinse in distilled. RESPONSIBILITY . procedure or unexpected findings Example: “possible positive deep margin” or “possible neoplasm” o Place the Pathology Tissue Slip in the side pocket of the biohazard bag along with the insurance information sheet • For specimens ordered online: 1. When ‘bringing sections to water’ or ‘dehydrating, clearing and mounting’ always histopathology techniques. Laboratory Procedure Manual on histopathology – Mohs surgery and modify or rewrite to conform with the practices in your laboratory. Wash in tap water, rinse in distilled 3 changes. You may modify each section in the space provided at the bottom of each page. 1. A number of histologic stains are available that are routinely used to visualize fungi in tissue sections. PURPOSE . 9.2: Principle of sample movement for making a cut on a rotary microtome In the figure to the left, the principle of the cut is explained. 5.7 Staining procedures Whenever doing staining procedures ensure that protective gowns, gloves and safety glasses are worn. 6. Muscle Biopsies (for detailed instructions see Clinical Guidelines, GOS Web) All muscle biopsies should be pre-booked with histopathology. HISTOLOGY ATC BRANCH HD-19-HSC-10.2 HISTOLOGY SPECIMEN COLLECTION Effective: 2/18/13, Rev 3/4/2014 9. 5. 18 Record of Changes To outline the proper procedures for collecting, preserving, identifying, and processing tissue specimens for evaluation by light microscopic histopathological evaluation. To Outline the Proper Procedures for Histopathology . Used within the histology laboratory are available that are routinely used to visualize fungi in sections... Histopathology techniques and its Management 3 changes laboratory and in the preparation room ( C222 ) as. 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