A Peripheral Blood Smear Examination is a procedure, which involves spreading a drop of blood thinly onto a glass slide. Without delay place a spreader at an angle of 45° from the slide and move it back to make contact with the drop. BLOOD COLLECTION BY VENIPUNCTURE – SYRINGE AND VACUTAINER METHOD. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. Your email address will not be published. The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. Why Edibles Affect Differently Than Smoking Marijuana. ⇒ Place the specimen glass slide on a flat surface and hold it with the index finger and the thumb of the left hand (for Right-handed peoples). Check out the Leishman staining protocol for staining blood smears, Check out the Giemsa staining Protocol for staining Blood Smears. A small drop of blood is placed on the midline at the end of a glass slide. and the Date on the frosted end of the slide. 3. All slides will be stained using Wrights stain or modified Wrights stain. ⇒ Now, Spread the blood evenly in a smear or film of about 10 mm wide and about the thickness through which you are able to read the words when the slide is placed on a printed paper. blood (within 1 hr. 2. The page below is a sample from the LabCE course. Peripheral Blood Smear Preparation. The Thick blood smears are prepared for detecting the Blood parasites such as Plasmodium spp. Each blood film should occupy three quarters of the length of the slide with clear areas surrounding the film, and with an oval feathered end. A) Preparation of Blood Smear Selection of a spreader: Take one slide a spreader which has smooth edge. Too slow a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end of the slide. On the other hand, Any condition which decreases the Packed cell volume or Hematocrit value like Anemia (Decrease in Red blood cell count) Bone marrow depression, etc. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Blood smears are mostly done for Differential Leukocyte count (DLC)i.e it quantifies the white blood cells and specifies the morphologies of each leukocyte. Phlebotomist may be required to prepare peripheral blood smears from capillary blood collected at the patient's bedside or from a drop of blood from an EDTA sample. The Thick blood smears are prepared for detecting the Blood parasites such as, PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD, MATERIALS REQUIRED FOR THIN PERIPHERAL BLOOD SMEAR METHOD, PROCEDURE FOR THIN PERIPHERAL BLOOD SMEAR METHOD, PRINCIPLE OF THICK PERIPHERAL BLOOD SMEAR METHOD. In this state, it Increases the angle between the spreader and the specimen glass slide to make a perfect blood film. Surgical Abortion vs Medical Abortion: Which One’s the Right Choice for You? The smear can then be stained as soon as it is dry, generally within 20-30 minutes of smear preparation. The drop should be in the center line of the glass slide. The peripheral blood film (PBF) is a laboratory work-up that involves cytology of peripheral blood cells smeared on a slide. Part 1 - Preparation of Peripheral Blood Smear (PBS) - YouTube A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. In this state, Decreases the angle between the Spreader and glass slide to obtain an ideal blood film. It should be done by careful look on the narrow edge of the slide or by moving a thumb smoothly on its edge. Other names: peripheral smear, peripheral blood film, smear, blood film, manual differential, differential slide, blood cell morphology, blood smear analysis The DLC involves the counting of 100 white blood cells (WBCs) noting the number, type, and characteristics of all the blood cells which aids the physician in diagnosing viral infections, bacterial infections, Parasitic infection and certain blood disorders such as Anemia, leukemia, bacterial or parasitic infection etc. Wear gloves. Hi, I’m the Founder and Developer of Paramedics World, a blog truly devoted to Paramedics. The site of examination is chosen; away from clumping, piling, or stacking of the red blood cells. Cannabidiol or CBD for Anxiety – Can Hemp Help You Calm Down? Place a small drop of blood, or one side about 1-2 cm from one end. Tnx for all keep it up am lab technician from Ethiopia. This article highlights the basic science and art behind the PBF. of collection) Preparation of Blood Film: The slide should be clean. ⇒ Let it stand for 2 minutes, the methanol present in the stain fixes the smear onto the glass slide. Now, Place a smooth, clean edge of the spreader slide on the specimen slide at an angle of about 30° – 45°. Thin smears. Many blood tests use computers to analyze results. Malaria Journal 2013; 12: 231. Fixation of the smear by methyl alcohol occurs in these 2 minutes The stain should not be allowed to dry Dilute with equal quantity of buffer solution (PH.6.8) or distilled water. For both methods, blood samples should be fresh and well-mixed at smear preparation time, and smears should be completely air-dried before staining. A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. The undiluted stain fixes and partially stains the smear. The blood smear in Figure 3C results when a slight amount of the blood droplet is … The blood smear is then fixed and stained with Romanowski stain for microscopic examination. This can most likely be observed at a site five or six oil fields from the end of the feathery portion (about 100 red cells per field). Reference: Norgan AP, Arguello HE, Sloan LM, Fernholz EC, Pritt BS. Keep the same angle all the way to the end of the smear. After staining the slide, the examination begins. Blood can also be taken from an EDTA sample.  Smears should be made within 1 hour of blood collection from EDTA specimens stored at room temperature to avoid distortion of cell morphology  Blood smears can also be made from finger prick blood directly onto slide. Blood smears are made by placing a well mixed drop of blood 1 to 2 mm in diameter 1/4 inch from the frosted edge of the slide. A well-prepared blood smear is important to produce good results on analysis after doing a Giemsa stain, in identifying blood cells or/and demonstrating the presence of parasites in a sample. associated with the decrease in the viscosity of blood. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. If you continue browsing the site, you agree to the use of cookies on this website. I am a Medical Lab Tech, a Web Developer and Bibliophiliac. Such an area for examination is illustrated in the image below. Preparation of Blood Smear: Collection of Sample: 1. Most commonly blood smears are used for the Differential Leukocyte count (DLC). Accurate assessment of the peripheral blood film begins with excellence in blood film preparation. CBC & Peripheral Blood Smear. Blood films are examined in the investigation of hematological disorders and are routinely employed to look for blood parasites, such as those of malaria and filariasis. The most common technique of blood smear preparation is called the “wedge or push” technique. This procedure is known as a blood film. This chapter describes techniques of preparation and interpretation of canine and feline blood smears and addresses integrating findings from blood smears with other parameters in CBCs. Slides should be air dried and stained within 2 hours after they are made. (Malaria parasite),  Wuchereria bancrofti & Brugia malayi (Lymphatic filariasis), Leishmania donovani or other species of Leishmania (Leishmaniasis), Babesia spp. Do not blow air on the slide from any source in an effort to enhance drying, it may distort the smear. There are many reasons why your doctor may order a blood smear. Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood sample from the vein in your arm. Place the Blood drop in the center of the glass slide. The drop should be in the center line of the glass slide. When done correctly, it should result in a uniform blood film, that gets progressively thinner. Take one drop of venous or capillary blood. Blood smear preparation is easy and inexpensive, and smear examination is readily learned with adequate background information and routine practice. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. The conditions like Polycythemia (increase in the Red blood cell count), Dehydration or any condition which increases the Packed cell volume or Hematocrit value is associated with the increase in the viscosity of blood. Start studying Preparation of Peripheral Blood Smear 3. Required fields are marked *. In this video I quickly go over how to make a peripheral blood smear. Preparation of a Peripheral Blood Film This protocol describes how to prepare a slide film of peripheral blood for microscopic examination. As previously mentioned in RBC abnormalities, one takes a venous blood sample, and then it is submitted for a CBC analysis \⠀洀慜ഀ挀栀椀渀攀尩, and another sample is submitted for a peripheral blood smear evaluation by smearing it on a glass slide. The blood sample is sent to the lab where a drop of blood is spread thinly onto a glass slide and it is then treated with a special strain. After staining the slide, the examination begins. Check out How to do a Capillary blood collection? Below, we discuss the procedures for preparing both thin and thick smear for Giemsa stainingtechnique, Importance, and applications of blood smears, in detail. 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