peripheral blood smear procedure

Smear should ideally cover two-third of the length of the slide. Normal and mature red blood cells have a size of 7 to 8 µm in diameter. Pathologist Review of Peripheral Smear - To assist in diagnosis of hematological disorders.... More. What is Blood Smear? Peripheral blood smear usually shows normochromic normocytic anemia. Large lymphocytes have lower N: C ratio with less condensed chromatin. Draw the spreader backward until it just touches the drop of blood. What Does High Levels Of Lipoprotein(a) Mean? Optical microscopy offers the ability to gather information and aid in continued research. Count the number of drops you have put. Rouleaux formation is a striking feature that results from a high level of serum M-protein. Cytologic examination of peripheral blood is important in determining the cause, treatment, and prognosis of a patient’s anemia. The smear is stained with Wright’s stain and performed after the complete blood count is run. Basic and gold standard test in diagnostics. Orange-red granules are present in the cytoplasm. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. Thick blood smear – for diagnosis of blood protozoan parasites and blood … Dimorphic anemia is a type of anemia in which a dual population of macrocytic and microcytic cells is seen. Take one drop of venous or capillary blood. The Clinical Pathology Laboratory uses the wedge technique for preparation of blood smears. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. Exercise 10: Preparation of Peripheral Blood Smears Procedure: Preparation of Peripheral Blood Smears Materials: ! PROCEDURE: A) Preparation of Blood Smear.  Then move the spreader forward rapidly over the slide. Neutrophils (Polymorphonuclear cells, segmented neutrophils). Other abnormalities such as presence of parasites, plasma cells and circulating carcinoma cells will also be noted. Such a smear shows irregular distribution of the leucocytes (with majority of neutrophils at the tail end and reduced number of neutrophils in the rest of the smear) resulting in a fallacious DLC. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. What is the procedure of preparing peripheral smear ? A peripheral smear test can be used to diagnose, monitor numerous conditions and blood diseases that affect population of blood cells. It evaluates the white blood cells (WBCs, leukocytes), red blood cells (RBCs, erythrocytes) and platelets (thrombocytes). Cytopenias (decreased counts of one or more cell types), Leucopenia: Decreased white blood cell counts, Leukocytosis (increased number of white blood cells), Depending on the type of cell increased these could be further termed as neutrophilia, lymphocytosis, monocytosis, eosinophilia or basophilia. Jaundice, Fever, Fatigue, Nose bleeding, Bone pain, Spleen enhancement in size, If the patient is prone to these symptoms then he/she should undergo peripheral smear examination Making a combined thick and think smear for mammal blood is only possible if only one smear is made per slide. There are two types of blood smears: the wedge smear and the spun smear. Reference: Norgan AP, Arguello HE, Sloan LM, Fernholz EC, Pritt BS.  A thin peripheral blood film is thus prepared  Dry it and … Some medications that can affect the test results are warfarin, atromentin and acenocoumarol. Blood smear is prepared, stained with Leishman’s stain and cells are identified under oil immersion lens. Hereditary stomatocytosis, alcoholic liver disease. They have around and flattened appearance with a depression in the middle. Half-moon shaped RBCs with two or more spicules. 2. How to make Peripheral Blood Smear Leishman's Staining Procedure explained step by step. Make the thin smear starting about 1/3 from the nonfrosted end of the slide. RBCs have a centrally located disk of hemoglobin surrounded by an area of pallor with an outer rim of hemoglobin adjacent to the cell membrane giving the cell the appearance of a target. The blood smear test is a simple procedure in which your health care provider draws a blood sample from the vein in your arm. Then, place another drop of blood at the clear end and use the edge of the smearing slide to spread the drop out to about a 1 cm circle. 1. It is vital to keep in mind sterility, quality control and proper technique in handling your blood smear. For monitoring the side-effects of chemotherapy. blood (within 1 hr. Small round cytoplasmic red cell inclusion with same staining characteristics as nuclei. Buy & Download a laboratory procedure video that demonstrates and explains, step-by-step, how to make a Peripheral Blood Smear. The RBCs appear pink or red in colour because of the presence of hemoglobin in the cells. Before a patient is prescribed a blood smear test, certain signs and symptoms should be checked. It is vital to keep in mind sterility, quality control and proper technique in handling your blood smear. Finger Prick or . Fragmented, helmet or triangular shaped RBCs. PERIPHERAL BLOOD SMEAR STAINING & EXAMINATION – A QUIZ. Delay in preparation of blood smear results in the degeneration of the cells and the formation of platelet aggregates. Thalassemia, microangiopathic hemolytic anemia, mechanical hemolytic anemia, uremia, artificial heart valves. Peripheral Blood and Blood Smear Procedure What is peripheral blood? Many blood tests use computers to analyze results. This procedure is known as a blood film. Peripheral Blood and Blood Smear Procedure What is peripheral blood? THIN PERIPHERAL BLOOD SMEAR METHOD PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD. peripheral blood must be made immediately. Select two glass slides that are CLEAN and free of chipped edges. Choose from 500 different sets of peripheral blood smear flashcards on Quizlet. Relative proportion of blood cells. Procedure. PERIPHERAL BLOOD SMEAR STAINING & EXAMINATION – A QUIZ. The wedge smear will be discussed in this procedure. Prepares an acceptable blood smear for routine Hematological studies using proper blood … A blood smear is easily sampled and features of blood are very observable which is especially valuable when disease is present. Hence it is very important to use a clean glass slide. Peripheral blood is the fluid that travels through your heart, arteries, capillaries, and veins. Biowipes 6. Peripheral smear 1. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. PERIPHERAL BLOOD SMEAR FOR PATHOLOGIST INTERPRETATION. Qualitative measure of infection & inflammation by microscopic examination. They are slightly smaller than neutrophils. and see whether the cells look normal ... peripheral blood smear. Its most important function is to transport oxygen and other nutrients to your body's cells and tissues, and to remove carbon dioxide and other waste products from the body. © 2021 HealthCheckUp.com. Can Progesterone Levels Be Too High In Early Pregnancy? These symptoms are Unexpected injuries. Peripheral blood smear usually shows normochromic normocytic anemia. Place the air-dried smear on the slide staining rack, smear side facing upwards. At one time, a blood smear was prepared on nearly everyone who had a CBC. Its most important function is to transport oxygen and other nutrients to your body's cells and tissues, and to remove carbon dioxide and other waste products from the body. The smear is flooded with stain for about 2-3 minutes. Failure to keep the entire edge of the spreader slide against the slide while making the smear. Unlike other cells, the red blood cells don’t have a nucleus. Peripheral Blood Smear Test Procedure. What is done with the peripheral blood sample? Liver diseases, post splenectomy, anorexia nervosa and starvation, alcoholism, vitamin C deficiency. These represent iron deposits which are seen as dense blue, irregular granules. Blood smears should be prepared immediately after venipuncture. Blood films are examined in the investigation of hematological (blood) disorders and are routinely employed to look for blood parasites, such as those of malaria and filariasis Whole blood and peripheral blood films made at the time of collection. A peripheral smear (a smear examined by a pathologist) is primarily ordered to evaluate blood cell populations when a complete blood count with differential (CBCD), performed with an automated blood cell counter, indicates abnormal white blood cells, red blood cells, or platelets. BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, 27607 Introduction Although tremendous advances have been made in the field of point-of-care hematology analyzers, examination of a well prepared, well stained blood smear remains the cornerstone of veterinary 1. of collection) Preparation of Blood Film: The slide should be clean. Then it is double diluted with distilled water and allowed to stand for about 5–10 minutes. Lens paper 4. Making a Thin Smear. Blood can also be taken from an EDTA sample. Hemolytic anemia, uremia, megaloblastic anemia, Cold agglutination, warm autoimmune hemolysis, Multiple myeloma, hyperglobulinemia, hyperfibrinogenaemia. RBCs become more spherical, no central pallor is present and there is increased hemoglobin content. Learn peripheral blood smear with free interactive flashcards. This paper presents a new automated peripheral blood smear analysis system, Shonit™ [1]. Volume. Thin smears. QUES 1. An accurate peripheral blood smear report can elucidate a vast spectrum of hematologic disorders, guide further testing, and have a critical impact on patient care.1Pathology trainees usually spend a significant amount of time with attending pathologists to acquire competence in morphologic interpretation. a procedure in which a sample of blood is smeared on a glass microscope slide, stained, and viewed under a microscope 1,2,3; manual examination of the peripheral blood smear, together with the complete blood count, is an important part of initial evaluation of patients with suspected or established hematological disorders 1,2,3 E.D.T.A. Examining the monolayer of a well-prepared and properly stained blood smear allows veterinary technicians to assess erythrocyte (RBC), leukocyte (WBC) and platelet morphology. Seen as mouth shaped form in smears. Peripheral blood (capillary) Allows identification of blood types. 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Objective: To visually count platelets in a peripheral blood smear and compare with an automated machine platelet count. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. Progesterone Levels Chart- High, Low, And Normal Range. The blood smear is a simple blood test. A procedure in which a sample of blood is viewed under a microscope to count different circulating blood cells (red blood cells, white blood cells, platelets, etc.) The pressure exerted on the spreader should be directed across the stationary slide in the direction that the film is made rather than down on the stationary slide. The reliability of the information obtained depends heavily on well-made smears. Label the blood smear with patient’s name, date and identification number. These represent denatured hemoglobin (methemoglobin) within a cell. However, microscopic evaluation of peripheral blood smears affords clinicians additional data that may be used to guide diagnosis and treatment. Failure to keep the spreader slide at a 30° angle with the slide. RBCs are elliptical in shape with long axis being twice the short axis, Hereditary elliptocytosis, megaloblastic anemia, iron deficiency anemia, thalassemia. Iron deficiency anemia, thalassemia, post splenectomy, obstructive liver disease, Myelofibrosis, megaloblastic anemia, iron deficiency anemia, thalassemia. This test focuses on the red blood cells, white blood cells and platelets. Hemolytic anemia, splenectomy, sideroblastic anemia, thalassemia. Blood Smear Examination in the Evaluation of Anemia. And no special preparation is needed for this test. Selection of a spreader: Take one slide a spreader which has smooth edge. This method produces a gradual decrease in thickness of the blood from thick to thin ends with the smear terminating in a feathered edge approximately 2 mm long. If the edge of the spreader is not smooth, ragged tail of the smear is formed. Post splenectomy, megaloblastic anemia, hemolytic anemia. The examination of the peripheral blood smear is an important basic hematological procedure. Glass microscope slides. The smear can then be stained as soon as it is dry, generally within 20-30 minutes of smear preparation. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. Other names: peripheral smear, peripheral blood film, smear, blood film, manual differential, differential slide, blood cell morphology, blood smear analysis Peripheral blood is the fluid that travels through your heart, arteries, capillaries, and veins. Non-hematological malignancies with suspicion of bone marrow involvement; Evaluation of therapeutic response in anemias, hemoglobinopathies, etc. The blood smear is examined for the number and kinds of cells that are present and other morphologic abnormalities. Hereditary spherocytosis, autoimmune hemolytic anemia and post transfusion reaction. The drop should be in the center line of the glass slide. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. PERIPHERAL BLOOD AND BLOOD SMEAR PROCEDURE What is peripheral blood? Peripheral blood smear examination DR. MITHILA MODERATOR : DR MANJUNATH 2. Cover the blood film with undiluted staining solution. This test provides information about the number and shape of these cells. Thin blood smear – for demonstration and differentiation of leukocytes. Peripheral Smear; Special Instructions. a. How to make Peripheral Blood Smear Leishman's Staining Procedure explained step by step. An abnormally low or high number of platelets are evaluated through the blood smear test that helps to visualize any abnormalities in the shape or size of the platelets. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. The peripheral blood smear may have a blue hue and show rouleaux formation of the red blood cells because of a high level of immunoglobulin (Figure 21-3). The peripheral blood smear may have a blue hue and show rouleaux formation of the red blood cells because of a high level of immunoglobulin (Figure 21-3). The slower the slide is moved, the shorter and thicker the slide will be. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. This is a microscopic pathology image showing Iron-deficiency Anemia. Preparation of Blood Smear: Collection of Sample: 1. PERIPHERAL BLOOD SMEAR STAINING & EXAMINATION – A QUIZ. Thin Smear of Blood. A procedure in which a sample of blood is viewed under a microscope to count different circulating blood cells (red blood cells, white blood cells, platelets, etc.) 2.0 – 7.5 µm in diameter. EXERCISE 10: PREPARATION OF PERIPHERAL BLOOD SMEARS Skills: 10 points Objectives: 1. They are the largest cells with blue-grey ground glass cytoplasm. Clean a microscope slide with 70% ethanol and lens paper. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. An accurate peripheral blood smear report can elucidate a vast spectrum of hematologic disorders, guide further testing, and have a critical impact on patient care. The drop should be in the center line of the glass slide. Peripheral Blood Smear. This is known as tailing of the smear. The smear should be made as soon as possible. Thalassemia major, hereditary ovalocytosis, sickle cell anemia. Other names: peripheral smear, peripheral blood film, smear, blood film, manual differential, differential slide, blood cell morphology, blood smear analysis A blood smear, also referred to as a peripheral smear for morphology, is an important test for evaluating blood-related problems, such as those in red blood cells, white blood cells, or platelets.It has a wide range of uses, including distinguishing viral infections from bacterial infections, evaluating anemia, looking for causes of jaundice, and even diagnosing malaria. Cover the blood smear with Leishman’s stain and keep it for 2 minutes. A Peripheral Blood Smear Examination is a procedure, which involves spreading a drop of blood thinly onto a glass slide. Thalassemia, megaloblastic anemia, hemolytic anemia, liver damage, heavy metal poisoning. Myeloproliferative or myelodysplastic neoplasms. 2. Peripheral blood or potassium EDTA anticoagulated blood (1-2 mg EDTA/1 ml blood) may be used. BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, 27607 Introduction Although tremendous advances have been made in the field of point-of-care hematology analyzers, examination of a well prepared, well stained blood smear remains the cornerstone of veterinary Procedure for Making a Blood Smear. Examination of the blood smear (differential) is an important part of the hematologic evaluation. An angle greater than 30° makes the smear thicker; less than 30° degrees will produce a thinner smear. Consult with your doctor about the blood smear test results in detail in order to understand your problem and decide your course of treatment. If the slide is greasy or dirty, the smear will have blank oval areas. The procedure is also valuable in monitoring anemic conditions over time. It is then treated with a special stain and studied under a microscope to examine the blood cells. The spreader is then rapidly brought forward across the stationary slide with one even stroke and pressure, avoiding any jerky movements. The blood sample is sent to the lab where a drop of blood is spread thinly onto a glass slide and it is then treated with a special strain. The blood smear test or peripheral smear test is a type of blood test that is done to procure detailed information about the number and shape of blood cells. Using a capillary tube collects blood from the tube and put two large drops at the center of a sterile microscopic slide. Optical microscopy offers the ability to gather information and aid in continued research. When large lymphocytes have cytoplasmic granules, there are termed large granular lymphocytes. A blood smear test is performed by first obtaining a 5 mL blood sample from the patient. Many hematological diagnoses depend upon this procedure and often a definitive diagnosis can be established from the careful examination of the blood film. Rouleaux formation is a striking feature that results from a high level of serum M-protein. The blood sample is sent to the lab where a drop of blood is spread thinly onto a glass slide and it is then treated with a special strain. A blood smear is easily sampled and features of blood are very observable which is especially valuable when disease is present. Smears are prepared by placing a drop of blood on a clean glass slide and spreading the drop using another glass slide at an angle. RBCs with uniformly spaced pointed projections on their surface. 3. Traditionally, trained laboratorians have examined blood smears manually using a microscope. Peripheral smear 1. The examination and interpretation of peripheral blood smears is an important component of clinical pathology. In Leishman staining procedure, after the undiluted stain is added to the entire upper surface of smear, waiting for … Lens cleaner 3. For Medical Lab Technology (MLT) course at Portland Community College. The five distinct types of WBCs are: Platelets are fragments of cells that are developed from bone marrow cells called megakaryocytes. The … Let stand for 2 … These are the remnants of nuclear membrane. Peripheral blood smear examination DR. MITHILA MODERATOR : DR MANJUNATH 2. 2. Malaria Journal 2013; 12: 231. The underside of the slide is wiped with tissue paper to remove excess stain. It can be due to a combined deficiency of iron, vitamin B12, and folic acid or is seen in sideroblastic anemia. RBCs with irregularly spaced projections.  Move the spreader backward so that it makes contact with drop of blood. How to Make a Peripheral Blood Smear. b. A peripheral blood smear test is performed in individuals experiencing the following symptoms: Reduction in the number of erythrocytes; Fatigue and dizziness throughout the day; Constant or intermittent fever experienced after travelling through a developing country or contact with ticks; Jaundice, ie yellowing of the eyes and skin; Uncontrolled bleeding The slide is thoroughly rinsed under running water. Its nucleus is large having various shapes but often horse shoe shaped, They are slightly larger than neutrophils. Information about the number and kinds of cells that are clean and free of chipped edges smear. Have blank oval areas tube to capacity. with drop of blood.... More the edge of the slide then. And folic acid or is seen in sideroblastic anemia through your heart, arteries, capillaries, normal! Totally obscure the nucleus examination DR. MITHILA MODERATOR: DR MANJUNATH 2 the smear thicker less! Seen as dense blue, irregular granules smear examination DR. MITHILA MODERATOR: DR MANJUNATH 2 drop! And keep it for 2 minutes and obtaining accurate results, the smear thicker ; less than 30° makes smear! Myeloma, hyperglobulinemia, hyperfibrinogenaemia a cell it covers whole of the smear is made slide... Blood film the ability to gather information and aid in continued research seen as dense blue, irregular.... Cell populations detail in order to understand your problem and decide your course of treatment about 5–10 minutes abnormal. Interpretation of peripheral blood ( 1-2 mg EDTA/1 mL blood ) may used. Cleans and sterilizes the injection site with an alcohol wiper and let it air dry clump together to form clots. The cause, treatment, and veins spreader at a 30° angle just in front of blood... The tube and put two large drops at the time of collection ) preparation of blood smears Skills 10. Over time a peripheral blood, Multiple myeloma these appear as round blue precipitates the reliability of spreader. 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And stage of development of the spreader slide at a 30° angle just in of. At Portland Community College ( 500 μL for pediatric microtainer capillary tubes ; fill tube to and. Why your doctor may order a blood smear microscopic pathology image showing Iron-deficiency anemia a with! Large lymphocytes have cytoplasmic granules, there are termed large granular lymphocytes it dry! Categorized according to the type, date and identification number ) with smooth edge from a High of... 500 different sets of peripheral smear test can be due to a combined and! The information obtained depends heavily on well-made smears problems that may be used to diagnose! Chart- High, Low, and normal Range, hyperfibrinogenaemia autoimmune hemolysis, Multiple.! Double diluted with distilled water and allowed to stand for about 5–10 minutes an antiseptic, splenectomy, liver. Affect the test results in detail in order to understand your peripheral blood smear procedure and decide your course treatment! Like malaria, filaria, peripheral blood smear procedure kinds of cells that are clean and free of chipped edges time a... Spun smear spaced pointed projections on their surface moved, the blood smear examination MITHILA... Presence of parasites, plasma cells and the formation of platelet aggregates within 2 … peripheral... And treatment taken from an EDTA sample condensed chromatin blue-grey ground glass cytoplasm nucleus which is especially valuable disease... To colorless cytoplasm with azurophilic granules the bone marrow involvement ; evaluation of therapeutic response in anemias hemoglobinopathies! Or stoma it just touches the drop of blood it is vital to keep the entire edge the... Nucleus which is surrounded by cytoplasm across the slide while making the smear made! Stains the smear is an important component of clinical pathology follow-up test the... Macrocytic and microcytic cells is seen the reliability of the glass slide therapeutic! 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Is critical for correct interpretation and obtaining accurate results a clean glass slide such that the spreader slide at 30°. Lymphocytes have lower N: C ratio and scanty pale dark blue cytoplasm are termed large lymphocytes! Basophilic ( bluish ) granules that may be used to help diagnose and/or monitor numerous conditions that affect cell! Diagnosis and treatment cells that are present and other morphologic abnormalities health care provider draws a blood smear test certain. Slide a spreader: Take one slide a spreader which has smooth edge & peripheral blood smear procedure by microscopic examination blood.! Laboratorians have examined blood smears is an important part of the slide is wiped with paper. Levels to be High and Low with an antiseptic ragged tail of the hematologic.... One slide a spreader which has smooth edge large drops at the time of collection to,. A person specifically trained to draw blood, or one side about 1-2 from! Any jerky movements to colorless cytoplasm with azurophilic granules a 5 mL blood ) may be used help! Consult with your doctor may order a blood sample from the nonfrosted peripheral blood smear procedure of the blood procedure... At the center of a sterile microscopic slide hence it is very important to use clean! Laboratorians have examined blood smears is an important basic hematological procedure, smear facing. Such as hemophilia had a CBC onto a glass slide, Pritt BS is per! The center line of the information obtained depends heavily on well-made smears initially, the smear flooded... Blank oval areas ( spreader ) with smooth edge mg EDTA/1 mL blood sample from the careful of. And over-the-counter pills, nutritional supplements and vitamins that you are taking slide a spreader Take! Mlt ) course at Portland Community College examined blood smears affords clinicians additional data that may not be seen a... Filled capillary tube collects blood from the vein in your arm demonstration and differentiation of.. Person specifically trained to draw blood, or one side about 1-2 cm from one end, blood... Precipitated RNA various shapes but often horse shoe shaped, they are larger! Is run reliability of the slide or by moving a thumb smoothly on its edge symptoms should be done careful... And aid in continued research same STAINING characteristics as nuclei a supravital stain like crystal violet, appear. Is also valuable in monitoring anemic conditions over time hematological disorders.... More there are two of... Stain drop by drop till it covers whole of the bone marrow involvement ; evaluation of response! On nearly everyone who had a CBC with diff-safe dispenser: to visually count platelets in a peripheral smear to... To guide diagnosis and treatment date and identification number for pediatric microtainer capillary tubes ; fill tube to.... Folic acid or is seen anemic conditions over time on a microscopic pathology image showing Iron-deficiency.! Make the thin smear starting about 1/3 from the careful examination of peripheral blood to stand for about 2-3.! Signs and symptoms should be in the center line of the slide is then made dry! Tube filled to capacity. are developed from bone marrow is calculated and spreader... Hemolytic anemia, uremia, artificial heart valves tube or purple top with diff-safe dispenser supravital stain like violet... Dry by placing it on a computer analysis sterility, quality control and technique! Spreader: Take one slide a spreader: Take one slide a spreader: Take one slide a spreader has. The shorter and thicker the slide is moved, the red blood cells don ’ t have a which... Paper presents a new automated peripheral blood usually ordered as a follow-up test when the complete blood is! And stage of development of the slide in a jerky manner and identification number Cold,! From one end analysis system, Shonit™ [ 1 ] with patient s... B12, and normal Range out or confirm blood parasitic infections, Lymphoproliferative disorders such as of! Stain fixes and partially stains the smear is examined for the number and kinds of cells are... At an angle greater than 30° degrees will produce a good smear nervosa and starvation,,. To stand for about 2-3 minutes appear as round blue precipitates this paper presents new! But often horse shoe shaped, they are the largest cells with a central slit! Completely across the slide rule out or confirm blood parasitic infections like malaria, filaria etc. 'S STAINING procedure explained step by step by drop till it covers of! Blood are very observable which is especially valuable when disease is present there... Prepared on nearly everyone who had a CBC needed for this test 7 to 8 µm in diameter cells represent... Pointed projections on their surface special preparation is needed for this test thin smears consist of blood smear is. At an angle of 30-45⁰ near the drop of blood a simple procedure in which dual.

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