A polychrome stain, contains methylene blue and eosine dye dissolved in methyl alcohol. 2. Understand the purpose of performing a peripheral blood smear examination. Desirable qualities of a blood smear include: i. BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, … Use either Push wedge method or Automated method to prepare blood smear. Clipping is a handy way to collect important slides you want to go back to later. Describe different erythrocyte inclusions, their etiology and their clinical significance. Improper timing of staining or buffering, Deteriorated reagent. 1. A peripheral blood smear is a glass microscope slide coated on one side with a thin layer of venous blood.The slide is stained with a dye, usually Wright’s stain, and examined under a microscope[1]. 2016 Dec 1;74(6):697-703 Authors: Wyrwas M, Michel J, Guibaud I, Lesesve JF, Bénet B Abstract The diagnosis of double hit lymphoma remains a challenge for the biologist for a good management of the patient. Normochromic when the color is normal. Sufficient reading area ADVERTISEMENTS: ii. The peripheral blood smear was consistent with a leukoerythroblastic pic-ture with normocytic Peripheral blood smear of a 38-year-old female with long-standing Crohn’s disease (CD) and development of microcytic anemia. Good Blood Smear - Margin free with distinct lateral edge, Smooth Appearance, Placed centrally on the slide, Has uniform distribution of leukocytes, Whole drop of blood is picked up and spread, Has gradual transition from thick to thin ,When held up to light has a rainbow appearance. A good wedge smear should cover half to three fourths of the slide, should show gradual transition from a thick to a thin end with a feathered edge. Pathophysiology There are various sizes and shapes of RBC seen in the peripheral blood smear like: Normocytic when the size is normal (7 to 8 µm). A peripheral smear test can be used to diagnose, monitor numerous conditions and blood diseases that affect population of blood cells. List the characteristics of a good blood smear Good Blood Smear - Margin free with distinct lateral edge, Smooth Appearance, Placed centrally on the slide, Has uniform distribution of leukocytes, Whole drop of blood is picked up and spread, Has gradual transition from thick to thin ,When held up to light has a rainbow appearance. 2. The blood smear demonstrated hypersegmented neutrophils (panels A,C), macrocytes (panel B), and red blood cell fragments (panels A,C). Ann Biol Clin (Paris). The smear shows numerous target cells and a spur cell (top right). Characteristics of A Good Smear 1. 11. 1. If you are using venous blood, blood smears should be prepared as soon as possible after collection (delay can result in changes in parasite morphology and staining characteristics). List the characteristics of a good blood smear. If you continue browsing the site, you agree to the use of cookies on this website. 3. A blood smear is used when a CBC with differential shows the presence of abnormal or immature cells. It might be used to help identify and/or keep an eye on numerous conditions Any condition which causes an increase in the Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood … Fingerstick- Best sample (no anticoagulant effect), 2. Cell Size (immature cells are larger), Nuclear to cytoplasmic ratio (relative and absoluate size of the nucleus decreases as a result of nuclear maturation) , RBC's (nucleus and other organelles disappear), Nucleus (Young nucleus is reddish and bcome bluer as cell ages, chromatin is fine and lacy and becomes coarse, clumped and condensed as cells mature), Cytoplasm (Granulocyte matures from non granulated to specific granules which differs in dye affinity, granules increase in number). It should have a smooth appearance and have no ridges, holes, lines, streaks or clumps. LIst the components of peripheral blood smear examination. Does not A. Peripheral In this video I quickly go over how to make a peripheral blood smear. Examining the monolayer of a well-prepared and properly stained blood smear allows veterinary technicians to assess erythrocyte (RBC), leukocyte (WBC) and platelet morphology. A lymphocyte is a type of white blood cell in the vertebrate immune system. 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